TmCalculator

This tool is extended from methods in Bio.SeqUtils.MeltingTemp of python. The melting temperature of nucleic acid sequences can be calculated in three method, the Wallace rule (Thein & Wallace (1986) doi:10.1016/S0140-6736(86)90739-7), empirical formulas based on G and C content (Marmur J. (1962) doi:10.1016/S0022-2836(62)80066-7, Schildkraut C. (2010) doi:10.1002/bip.360030207, Wetmur J G (1991) doi:10.3109/10409239109114069, Untergasser,A. (2012) doi:10.1093/nar/gks596, von Ahsen N (2001) doi:10.1093/clinchem/47.11.1956) and nearest neighbor thermodynamics (Breslauer K J (1986) doi:10.1073/pnas.83.11.3746, Sugimoto N (1996) doi:10.1093/nar/24.22.4501, Allawi H (1998) doi:10.1093/nar/26.11.2694, SantaLucia J (2004) doi:10.1146/annurev.biophys.32.110601.141800, Freier S (1986) doi:10.1073/pnas.83.24.9373, Xia T (1998) doi:10.1021/bi9809425, Chen JL (2012) doi:10.1021/bi3002709, Bommarito S (2000) doi:10.1093/nar/28.9.1929, Turner D H (2010) doi:10.1093/nar/gkp892, Sugimoto N (1995) doi:10.1016/S0048-9697(98)00088-6, Allawi H T (1997) doi:10.1021/bi962590c, Santalucia N (2005) doi:10.1093/nar/gki918), and it can also be corrected with salt ions and chemical compound (SantaLucia J (1996) doi:10.1021/bi951907q, SantaLucia J(1998) doi:10.1073/pnas.95.4.1460, Owczarzy R (2004) doi:10.1021/bi034621r, Owczarzy R (2008) doi:10.1021/bi702363u).