CRAN/E | AmpliconDuo

AmpliconDuo

Statistical Analysis of Amplicon Data of the Same Sample to Identify Artefacts

Installation

About

Increasingly powerful techniques for high-throughput sequencing open the possibility to comprehensively characterize microbial communities, including rare species. However, a still unresolved issue are the substantial error rates in the experimental process generating these sequences. To overcome these limitations we propose an approach, where each sample is split and the same amplification and sequencing protocol is applied to both halves. This procedure should allow to detect likely PCR and sequencing artifacts, and true rare species by comparison of the results of both parts. The AmpliconDuo package, whereas amplicon duo from here on refers to the two amplicon data sets of a split sample, is intended to help interpret the obtained read frequency distribution across split samples, and to filter the false positive reads.

Key Metrics

Version 1.1.1
R ≥ 2.10
Published 2020-05-25 1215 days ago
Needs compilation? no
License GPL (≥ 3)
CRAN checks AmpliconDuo results

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Maintainer

Maintainer

Anja Lange

anja.lange@uni-due.de

Authors

Anja Lange

aut / cre

Daniel Hoffmann

aut

Material

Reference manual
Package source

macOS

r-release

arm64

r-oldrel

arm64

r-release

x86_64

r-oldrel

x86_64

Windows

r-devel

x86_64

r-release

x86_64

r-oldrel

x86_64

Old Sources

AmpliconDuo archive

Depends

R ≥ 2.10
stats
ggplot2
xtable